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Objective:To investigate the clinical value of peripheral monocyte and neutrophil count in predicting the response of patients with metastatic non-small cell lung cancer (mNSCLC) to immunosuppressive checkpoint inhibitors (ICI).Methods:The clinical data of 34 adult mNSCLC patients who received nafulizumab or pabolizumab in Danzhou People's Hospital of Hainan Province from January 2017 to March 2019 were retrospectively analyzed. The correlation of the demographic characteristics, clinical data, hematological examination results in the first two weeks before the treatment and two weeks after ICI treatment with prognosis was recorded and observed.Results:The baseline mean monocyte count [(0.52±0.09)×10 9/L vs. (0.60±0.12)×10 9/L] and neutrophil count [(4.27±0.87)×10 9/L vs.(5.39±1.02)×10 9/L] of patients with ICI reaction were lower than those of patients without ICI reaction, and the differences were statistically different ( t = -2.572, -2.727, all P < 0.05). However, there was a negative correlation between the monocyte count of the patients who responded to ICI and the reaction time ( r = -0.507, P < 0.05). The median reaction time in patients with monocyte count >0.70×10 9/L was shorter than that in patients with monocyte count ≤0.70×10 9/L (8 weeks vs. 12 weeks, χ2=4.162, P = 0.041). There was no correlation between monocyte count and time of reaction duration, progression of free survival (PFS) and overall survival (OS) ( r = -0.214, 0.182, 0.232, all P > 0.05). The decrease rate of neutrophil count in response group was higher than that in non-response group (22% vs. 2%, P < 0.05). After the first administration, cutoff value of neutrophil count was 4.2×10 9/L; the response rate of patients with neutrophil count ≤ 4.2×10 9/L was higher than that of patients with neutrophil count > 4.2×10 9/L [86.7% (13/15) vs. 36.8% (7/19), χ2=6.657, P < 0.05]. Conclusion:Peripheral blood monocyte and neutrophil count can predict the response to ICI therapy in patients with mNSCLC.
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Lung cancer is currently one of the most common malignant tumors in the world. The occurrence and development of lung cancer, especially non-small cell lung cancer (NSCLC), are closely related to the abnormal expression of long non-coding RNA (lncRNA). lncRNA with a transcript of more than 200 nucleotides is involved in chromatin modification, transcription activation, transcription interference and other regulatory processes, and has varying degrees of regulation on the proliferation, migration, and invasion of tumor cells. It is characterized by up-regulation or down-regulation of expression. At present, there are a large number of studies on lncRNA, because lncRNA has considerable application prospects in the diagnosis, clinical treatment, drug resistance research and prognosis evaluation of NSCLC. In this paper, the overview of lncRNA, the up-regulation or down-regulation of NSCLC-related lncRNA expression, NSCLC clinical treatment and drug-resistant lncRNA were summarized.
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Objective@#To describe job satisfaction of paediatric intensive care units in China, and analyze the relationship of nursing work environment between job satisfaction.@*Methods@#From November 2017 to May 2018, a total of 1 414 nurses of paediatric intensive care units from 25 hospitals of 16 provinces and cities nationwide in China were selected using convenient sampling, and surveyed by Nurses Job Satisfaction Scale and Practice Environment Scale. Descriptive statistics and Hierarchical multiple regression analysis were performed by SPSS 20.0.@*Results@#The average score of nurse job satisfaction of paediatric nurses was 3.29 ± 0.49, which was in the middle level in China. Hierarchical regression results showed that the area where the hospital was located, monthly income could affect the nurse job satisfaction, and nursing work environment played an important role for nurses job satisfaction, the model explained 73.1% amount of variance (R2=0.731, P<0.01).@*Conclusions@#The nursing work environment is the important factor influencing the nurses job satisfaction of paediatric intensive care unit, how to balance the domestic resources and reduce the regional differences, we work together to create a healthy environment in paediatric intensive care unit, further improving the nurses job satisfaction will be the important issues in the future.
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Objective To describe job satisfaction of paediatric intensive care units in China, and analyze the relationship of nursing work environment between job satisfaction. Methods From November 2017 to May 2018, a total of 1414 nurses of paediatric intensive care units from 25 hospitals of 16 provinces and cities nationwide in China were selected using convenient sampling, and surveyed by Nurses Job Satisfaction Scale and Practice Environment Scale. Descriptive statistics and Hierarchical multiple regression analysis were performed by SPSS 20.0. Results The average score of nurse job satisfaction of paediatric nurses was 3.29 ± 0.49, which was in the middle level in China. Hierarchical regression results showed that the area where the hospital was located, monthly income could affect the nurse job satisfaction, and nursing work environment played an important role for nurses job satisfaction, the model explained 73.1％ amount of variance (R2=0.731, P<0.01). Conclusions The nursing work environment is the important factor influencing the nurses job satisfaction of paediatric intensive care unit, how to balance the domestic resources and reduce the regional differences, we work together to create a healthy environment in paediatric intensive care unit, further improving the nurses job satisfaction will be the important issues in the future.
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Objective@#To analyze the epidemiological characteristics and distribution characteristics of tick-borne encephalitis in China in 2014, and to provide scientific basis for formulating specific prevention and control measures.@*Methods@#The epidemic data were obtained from the "infectious disease report information management system" , using Excel 2016, GIS and other software to summarize and analyze the cases of tick borne encephalitis (TBE) reported, using the number of cases, incidence, composition ratio and other indicators to analyze and describe the TBE epidemiological characteristics in China in 2014.@*Results@#In 2014, a total of 322 cases of TBE were reported in 9 provinces in China, with an annual incidence of 0.024/100, 000 and 1 death of patient. The provinces with high number of cases were Jilin province, Inner mongolia autonomous region and Heilongjiang province, and the number of cases in the other six provinces is no more than two. TBE was distributed in spring and summer, and it is concentrated in May to July. The age of the affected population was mostly concentrated in 40-49 years old, the male-female ratio was 1.6∶1 (198/124), and the patients were dominantly farmers, household and unemployed workers, and forestry workers, they accounted for 49.40% (159/322), 26.40% (85/322) and 18.60% (60/322) of the national TBE cases respectively. The three hospitals that reported the most TBE cases in 2014 were Inner mongolia forestry general hospital, Jiangyuan People′s hospital of Baishan city, Jilin province and Mudanjiang forestry central hospital of Heilongjiang province. The number of reported cases in these three hospitals accounted for 68.6% of the whole country. The laboratory diagnosis rate of Inner mongolia forestry general hospital was the highest (91.9%).@*Conclusions@#In 2014, the incidence of TBE in China has continued to rise compared with the previous two years. The geographical focus is mainly on the forest areas of Daxing′anling, Xiaoxing′anling and Changbai Mountain.
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Objective@#In this study, the mRNA of interferon (IFN)-α, IFN-γ, Mov10 and TRIM22, which are innate immune factors, was detected in chronic hepatitis B patients with nucleoside analogues multidrug resistance to investigate host innate immune status and explore the host immune molecular mechanism under the nucleos(t)ide analogues multiresistant HBV infection.@*Methods@#Twenty eight nucleos(t)ide analogue resistant patients and 20 healthy individuals in the control group were collected from infection medical clinic department of Heilongjiang provincial hospital. There were 18 cases of multiple drug resistance and 10 cases of cross resistance.Their peripheral blood specimens were collected. The mRNA of IFN-α, IFN-γ, Mov10 and TRIM22, that are the innate immunity factors in peripheral blood, were detected in 28 nucleos(t)ide analogue resistant patients and 20 healthy individuals using RT-PCR and the data were analyzed by statistical mthods.@*Results@#The mRNA levels of Mov10 and TRIM22 mRNA that are innate immune factors in peripheral blood mononuclear cells of multidrug resistant chronic hepatitis B patients were higher than those of control groups with statistically significant differences (P<0.01). TRIM22 mRNA in chronic hepatitis B patients with multidrug resistance showed positive correlation with IFN-α, IFN-γ and Mov10 mRNA; Mov10 mRNA showed positive correlation with TRIM22 and IFN-α mRNA; IFN-α mRNA showed positive correlation with IFN-γ, Mov10 and TRIM22 mRNA. There were no statistically significant differences in the innate immunity factors IFN-α, IFN-γ, Mov10 and TRIM22 mRNA between multidrug resistant group and cross-resistant group.@*Conclusions@#The mRNA levels of Mov10 and TRIM22, that are innate immune factors, were higher in chronic hepatitis B patients with nucleos(t)ide analogues resistance than those of healthy control group. The correlation analysis of mRNA levels between IFN-α, IFN-γ, Mov10 and TRIM22 expression demonstrated that the correlations among them were different under the nucleoside analogues resistant HBV infection.
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Objective:To review the new progress in hydrogen peroxide disinfection and sterilization technology and equipments. Methods:Based on the recently published papers and our research results,the hydrogen peroxide sterilization technology and equip-ments were classified and summarized. Results:The hydrogen peroxide nano-fog( ultra-dry fog) sterilization technology and equipments could be used for the sterilization of space and equipments with the properties of safety, effectiveness and compatibility. Conclusion:The nano-fog( ultra-dry fog) sterilization technology and equipments exhibit broad application prospects,which is an ideal method to re-place the conventional space sterilization using formaldehyde and ozone.
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@# Objective To explore the effect of Tuina combined with treadmill training on nerve regeneration after sciatic nerve transection. Methods Ninty-six Sprague-Dawley rats were randomly divided into normal group (n=32), model group (n=32) and treatment group (n=32). The sciatic nerve was transected and the epineurium was sutured in the latter two groups. The treatment group was given Tuina manipulation and treadmill training once a day. Eight rats from each group were detected their sciatic nerve conduction velocity (NCV), the number of axons and Schwann cells (SCs) two, three, four and eight weeks after intervention. Results Compared with the model group, the NCV accelerated in the treatment group four and eight weeks after intervention (P<0.05); while the number of axons was significantly different two and four weeks after intervention (P<0.05), and the number of SCs was not very different between the treatment group and the model group after intervention (P>0.05). Conclusion Tuina combined with treadmill training may promote the regeneration of peripheral nerve after injury in rats.
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Objective The study aimed to construct one severity of illness scoring system specially for children with complex congenital heart diseases and analyzed the applicability of this new score,which will be helpful to assist the medical workers in evaluating children's condition.Methods A total of 1284children patients with complex congenital heart diseases in Shanghai Children's Medical Center Affiliated to School of Medicine of Shanghai Jiaotong University.The general information and clinical data were collected.Logistic regression,method of percentiles and expert opinions were used to construct the new severity of illness scoring system.Area under ROC curve,Hosmer-Lemeshow,goodness of fit test were used to evaluate the performance and applicability of the new score.Results A total of 1284 children with complex congenital heart diseases split into construction group (1138 cases) and verification group (146 cases).The new score included seven indexes (systolic blood pressure,blood oxygen saturation,lactic acid,potassium,potential of hydrogen,glucose,positive inotropic drug score).The range of the score was 0-57 and the cut-off point was 10.The discriminatory power was sufficient (area under the curve ＞ 0.75),and the calibration capability of the score was tolerable (P ＞ 0.05).Conclusions The applicability of the new score is well and it is easy to use,which will not increase the workload for the medical workers while assisting them in the clinical environment.
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BACKGROUND:Previous studies have performed three-dimensional finite element analysis on lumbar fracture fixation. However, a few studies concerned three-dimensional finite element analysis of lumbar spinal pedicle screw fixation. OBJECTIVE:To analyze the biomechanical characteristics of one-way screw and universal screw fixation for lumbar fractures using finite element method. METHODS:Models of lumbar fractures treated with one-way screw and universal screw fixation were established. Three-dimensional finite element method was used to analyze and compare the maximum axial displacement, maximum bending degree, maximum stress on thepedicle screw fixation system and maximum stress of intervertebral disc in normal vertebral fracture model, one-way and universal pedicle screw fixation models. RESULTS AND CONCLUSION:(1) The maximum axial displacement at anteflexion, posterior extension, left lateral curvature and right lateral curvature was significantly lower in the one-way fixation group and universal fixation group than in the normal group (P< 0.05-0.01). The maximum axial displacement at above directions was lower in the universalfixation group than in the one-way fixation group. (2) The maximum bending degree was significantly lower in the one-way and universal fixation groups than in the normal group (P< 0.05-0.01). The maximum bending degree at various directions was lower in the universal fixation group than in the one-way fixation group. (3) The maximum stress on the pedicle screw fixation system at various directions was lower in the universal fixation group than in the one-way fixation group (P< 0.05-0.01). (4) The maximumstress of intervertebral disc was significantly lower in the one-way and universal fixation groups than in the normal group (P< 0.05-0.01). The maximum stress of intervertebral disc at various directions was lower in the universal fixation group than in the one-way fixation group. (5) Results suggested that one-way and universal screw fixation for lumbar fracture can bear most of the stress, obviously decrease the maximum axial displacement, maximum bending degree, maximum stress on the pedicle screw fixation system and maximum stress of intervertebral disc. The stability of universal screw fixation was better than that of one-way screw fixation.
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OBJECTIVE To investigate the effect of tranilast on cardiac fibroblasts proliferation and phenotype transformation incubated with a high concentration of glucose and the possible mechanism. METHODS The cardiac fibroblasts were divided into seven groups in accordance with different nutrient solutions:normal control group(5.5 mmol · L-1 glucose),hypertonic group(5.5 mmol · L-1 glucose+mannitol 25 mmol · L- 1),high glucose group (25 mmol · L- 1 glucose),tranilast intervention groups (25 mmol·L-1 glucose+tranilast 50,100 and 200μmol·L-1),and activin receptor-like kinase 7(ALK7) inhibitor group(25 mmol · L-1 glucose+10μmol · L-1 SB431542). The cell proliferation was detected by MTT method. The transformation of cardiac fibroblasts was determined by immunfluorescence staining. The expression of fibroblast specific protein 1 (FSP-1),α-smooth muscle actin (α-SMA),transforming growth factor-β1(TGF-β1)and ALK7 was assessed by Western blotting. RESULTS Compared with nor?mal control group,A492 nm of cells in high glucose group was significantly increased(P<0.01),while the expression of α-SMA,TGF-β1 and ALK7 protein in high glucose group was significantly increased(P<0.01),but FSP-1 protein was significantly decreased(P<0.01). There was no difference between normal control group and hypertonic group. Compared with high glucose group,A492 nm of cells in tranilast or SB431542 intervention groups were decreased(P<0.05),and the expression of α-SMA,TGF-β1 and ALK7 protein was significantly decreased(P<0.05),but the expression of FSP-1 protein was increased (P<0.05)in tranilast or SB431542 intervention groups. CONCLUSION Tranilast can inhibit the proliferation and phenotype transformation of cardiac fibroblasts induced by high glucose,which may be related to down-regulation of the expression of ALK7.
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Objective To investigate the association of hepatitis B virus ( HBV) precore ( preC) /C mutations with the pro-gression of liver disease .Methods The HBV genes of 50 chronic hepatitis B , including 30 HBV e-antigen ( HBeAg)-negative and 20 HBeAg-positive patients, were detected with real-time quantification polymerase chain reaction (RT-PCR) and the direct sequencing method.Results The mutations T1762/A176, T1766/A1768, A1896 , and the levels of HBV viral loads significantly correlated with the disease progression .The HBeAg-negative patients had a higher frequency of mutations at T 1762/A1764 , T1766/A1768 , and A 1896 relative to HBeAg-positive patients .Conclusions Patients with advanced liver diseases and with HBeAg-negativity possibly had multi-mutations at T1762/A1764, T1766/A1768, and A1896 in HBV genomes.
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Objective To investigate the current status of postoperative life quality of 2-to 12-yearold children with congenital heart disease,to compare results between 2-to 4-year-old,5-to 7-year-old and 8-to12-year-old groups.Meanwhile,compare the results with foreign researches.Methods Pedsol cardiac module was adopted to investigate 255 children patients with letters in a certain heart center in Shanghai.The survey included heart problems and treatment,perceived physical appearance,treatment anxiety,cognitive problems and communication.2-to 4-year-old group was reported by parents and others were reported by both children and parents.The results underwent analysis and were compared with data of other countries.Results There existed statistical difference among three groups.Compared with foreign researches,quality of life of Chinese children was lower except heart problems and treatment.Conclusions We should provide suitable health care and family centered nursing according to physical and psychological development of children to improve children's quality of life.
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ObjectiveTo observe and evaluate the efficacy of Clostridium Butyricum Powder for preventing childhood diarrhea after pneumonia. Methods176 infants with pneumonia were randomly divided into two groups,100 infants as prevention group, another 76 infants as control group. With normal antibacterial therapy,and two hours afterwards. Clostridium Butyricum Powder was administered at doses of 0.5g three times a day,in prevention group.After diarrhea taken place, Clostridium Butyricum Powder was administered at doses of 0. 5 g three times a day, in control group. Clinical effect and untoward effect were observed. ResultsThe incidence of pneumonia-induced diarrhea was 9% in prevention group,and was 46. 8% in control group( P < 0. 01 ). Duration of diarrhea and course of the treatment in prevention group as compared to those in the control group were statistically significant( P < 0. 01 ).Adverse reaction was not found. ConclusionClostridium Butyricum Powder can prevent diarrhea after pneumonia with antibacterial therapy.
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@#ObjectiveTo observe the effect of comprehensive rehabilitation on chronic schizophrenic inpatients. Methods200 chronic schizophrenics were divided into study group and control group, BPRS and IPROS were used in evaluation before and 3-week after training. ResultsThe scores of both BPRS and IPROS in study group significantly improved (P<0.01) after rehabilitation; only the score of BPRS in control group improved (P<0.05) in control group. ConclusionComprehensive rehabilitation can improve the social function in patients with chronic schizophrenia.
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Objective To explore the value of strain rate imaging (SRI) technology in the diagnosis of hypertension patient with re-gional left ventricular early diastolic dysfunction. Methods 50 essential hypertension patients were divided into hypertension Group Ⅰ and Group Ⅱ, including 31 patients with normal left ventricular and 19 patients with left ventricular hypertrophy. 25 healthy people were used as the control group. SRI was done in the six different basal myocardial segments and the parameters of SRI were compared with that of Doppler tissue imaging (DTI) and pulse Doppler (PW). Results In the hypertension group, SRe of the six different basal myocardial segments was lower than that in the control group (P<0.05), but (SRa) of hypertension group was higher (P<0.05). SRe was negatively correlated with the patients'illness development (r=-0.89, P<0.05), but SRa was positively correlated (r=0.97, P<0.05). And the SRe/SRa<1 detection rate of Group Ⅱ was higher than that in Group Ⅰ, and the ratio in the control group was lowest(P<0.05). Total detection rate of SRI (88%) was higher than Em/Am <1 of DTI (79.67%) and E/A < 1 of PW(60%), and these changes were more obvious in Group Ⅰ. SRI was higher than DTT and PW (P<0.05). Conclusion Compared with traditional technology and methods, SRI could pro-vide more accurate diagnoses to hypertension patient with regional left ventricular early diastolic dysfunction.
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BACKGROUND: Oxygen-derived free radicals are produced during non-enzymatic glycosylation of diabetic protein and accompanied with decrease in nitrogen monoxide (NO) synthesis so as to cause the calcium increase in cell,evacuation of pykno-granule and apoptosis induced by activating endoenzyme.OBJECTIVE: To observe the effects of non-enzymatic glycosylation inhibitor-aminoguanidine on apoptosis of cardiac myocyte and cardiac function in diabetic rats.DESIGN: Completely randomized grouping design and controlled study.SETTING: Pharmacological Department of Jinzhou Medical College.MATERIALS: The experiment was completed at the Central Laboratory of Jinzhou Medical College between September 2002 and March 2003. Totally 54 male SD rats with 2-month old were selected.METHODS: Totally 36 rats were selected to establish diabetic model 60 mg/kg of streptozotocin were injected into the caudal vein. If blood glucose of rats was more than 16.7 mmol/L, the establishment of diabetic model was successful. Model rats were divided into diabetes group and aminoguanidine (AG) group with 18 in each group. Rats in each group were also divided into two 12-week groups with 8 and 12 respectively. Another 18 rats were determined as the control group at 2 time points: 12 weeks (n=8) and 24 weeks (n=10). Rats in each group were fed for 12 and 24in other two groups. Calculation of mass index was [heart (mg)/body mass (g)]. Myocardial tissue of left ventricle was taken out and observed with transmission electron microscope and then stained with in situ end-labeling (ISEL) method. Number of positive nucleus was counted with 10 × 10 ocular lens check system and with 10 fields ISEL method; meanwhile, their average was obtained.MAIN OUTCOME MEASURES: Whether there was apoptosis of cardiac cell and the effect on AG in changes of cardiac structure and function of diabetic rats or not.RESULTS: Eight rats were lost during the experiment because of death mass: That of rats in the 12-week and 24-week diabetic group was higher decrease and increase rate of pressure in left ventricle: That of rats in the 12-week and 24-week diabetic group was lower than that in the control in left ventricle: That in 24-week diabetes group was obviously lower than diabetic group was obviously more than that in AG group (P ＜ 0.01), and that in 24-week diabetes group was obviously more than that in 12-week Apoptosis could be observed in myocardial cell in diabetic group.CONCLUSION: Apoptosis of myocardial cell plays an important role in the development of heart failure in diabetic rats. AG can reduce the apoptosis of myocardial cell and decrease the myocardial pathomorphological abnormality.
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Aim To study the effects of pioglitazone on the hypertrophic myocardial cells induced with high glucose and high insulin. Methods Using cultured myocardial cells as a model, The protein content was assayed with Lowrys method; The contracting frequency was counted by the inverted microscope;The protein synthesis was assayed with leucine intake method;The cardiomyocytes volumes was measured by computer photograph analysis system; the cellular hypertrophy was observed. Results Compared with verapamil, Pioglitazone significantly inhibited the protein content, cellular protein synthesis and volumes of cultured hypertrophic myocardial cells induced with high glucose and high insulin. Meanwhile pioglitazone and verapamil inhibited myocardial cells contracting frequency too. Conclusions Pioglitazone inhibited cardiac hypertrophy of cultured myocardial cells induced with high glucose and high glucose. The mechanism of the effect of pioglitazone on cardiac hypertrophy needs further study.
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Objective To investigate the change of eNOS,the spermatogenic cell proliferation,apoptosis in mouse testis exerted by alcohol. Methods The immunohistochemical staining method for detecting of eNOS,PCNA,TUNEL method for detecting of apoptotic cells and the satistics analysis were used in the present study. Results With the increase of alcohol concentration,the structure of seminiferous tubule changed,the diameter of seminiferous tubule decreased,the surface density of postive eNOS cells increased gradually,and the number of positve PCNA cells and apoptosis cells also increased.There were significant difference in 15% alcohol concentration group compared with the other groups(P
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The history of life sciences has displayed that Interdisciplinary contributes to the development of life sciences.It also causes people to think dialectically,what is regarded as the development of Interdisciplinary,what is its drive causes,what is the rule of its development.By exploring the developing trend of scientific and interdisciplinary science,we have fully discussed how to train the Cross-talents.